Universal Nuclease (Ben Nuclease), also known as non-restrictive endonuclease or broad-spectrum nuclease, is an engineered nuclease derived from Serratia marcescens. It is capable of degrading all forms of DNA and RNA, including double-helical, single-stranded, double-stranded, linear, circular, natural, and denatured nucleic acids, digesting them into fragments of 3-5 nucleotides in length with a 5'-monophosphate terminus. It functions effectively under various operational conditions and does not require specific nucleic acid base sequences, making it capable of acting at any nucleotide position within the chain.
