Magose His-tag protein purification magnetic beads are Ni2+ ions immobilized agarose magnetic beads, capable of reversibly binding with histidine labeled proteins or other proteins that can specifically bind to Ni2+, so as to obtain target proteins by one-step purification. The product is convenient to use,simplifying purification process significantly and reducing equipment requirements,can be used for fast and efficient purification of small quantities, as well as for high-throughput screening.