BHK SFM01 medium is a serum-free culture medium that contains no serum or animal-derived components. It is suitable for the suspension adaptation and large-scale serum-free suspension culture of BHK cells, and the cultivation process does not require the addition of serum. It supports rapid proliferation, high-density cultivation of BHK cells, and virus vaccine production in a completely serum-free and protein-free system. It can be used in the research and production processes of vaccines such as rabies vaccines and can support the entire spectrum from research to large-scale manufacturing.