MDCK SFM01 is a serum-free culture medium that is entirely devoid of serum and contains no animal-derived components. It is suitable for the suspension adaptation and large-scale serum-free suspension culture of MDCK cells, and there is no need to add serum during the cultivation process. It supports the rapid proliferation, high-density cultivation, and influenza virus vaccine production of MDCK cells in a completely serum-free and protein-free system. It can be used in the research and production processes of products related to influenza vaccines and can support the entire spectrum from research to large-scale manufacturing.