Insect SFM01 medium is a serum-free culture medium devoid of any serum or animal-derived components. It is suitable for the suspension adaptation and large-scale serum-free suspension culture of insect cells (such as Sf9, High Five), enabling rapid high-density proliferation and efficient protein expression. It can be employed in the development and production processes of products like COVID-19 vaccines and VLPs, supporting the entire spectrum from research to large-scale manufacturing.