GST is a commonly used tag to purify recombinant proteins which provides a high purity in one-step purification. Sometimes, GST-tag can improve the solubility and stability of target proteins. However, the tag is often cleavage after purification. Because GST is a large protein tag and easily forms a dimer under normal purification conditions. GSH Purose 4 Fast Flow is design to purify glutathione S-transferase (GST)-tagged proteins. The purification process is mild which is important to keep protein function and antigenicity stable.